Metagenomics analysis for microbial ecology investigation on historical samples: negligible effect of host DNA and optimal analysis strategies

This study demonstrates that host DNA removal is unnecessary for analyzing historical microbiome samples and proposes an optimized two-step k-mer classification strategy that effectively recovers microbial signals from fragmented DNA, thereby enabling the integration of natural history collections into long-term ecological research.

Ng, S.-K., Gutaker, R.2026-03-25📄 plant biology

A three amino acid sequence gates protein stability control of bHLH104 and iron uptake in Arabidopsis thaliana

This study demonstrates that the C-terminal proline-alanine-alanine (PAA) motif in the Arabidopsis transcription factor bHLH104 is essential for its interaction with the E3 ligase BTS and subsequent ubiquitination-mediated degradation, as its removal leads to protein stabilization, constitutive upregulation of iron acquisition genes, and increased iron accumulation.

Baby, D., Weldi, S., Knopf, M. + 3 more2026-03-25📄 plant biology

Larval antibiosis to cabbage stem flea beetle (Psylliodes chrysocephala) is absent within oilseed rape (Brassica napus)

This study demonstrates that while larval antibiosis against the cabbage stem flea beetle is absent in oilseed rape (*Brassica napus*), likely due to domestication bottlenecks, it is present in *Sinapis alba* and model species like *Brassica rapa* and *Arabidopsis thaliana*, offering new avenues for resistance breeding.

Brock, R. E., Courtney, C., Penfield, S. + 1 more2026-03-25📄 plant biology

Reference genome assembly of a tetraploid accession of the tuber crop Tropaeolum tuberosum

This study presents a high-quality, near-complete reference genome assembly of a tetraploid *Tropaeolum tuberosum* accession generated via PacBio HiFi sequencing, which was validated for accuracy and broad applicability to diverse germplasm, thereby establishing a foundational resource for future breeding, conservation, and functional genomic research on this underutilized Andean tuber crop.

Ruiz-Mateus, D., Scheffler, I., Marquez-Cardona, M. d. P. + 3 more2026-03-25📄 plant biology

Presymptomatic plant disease detection with PSNet: A low-cost hyperspectral imaging and RGB fusion framework.

This paper introduces PSNet, a low-cost multimodal framework combining a 3D-printed hyperspectral camera with RGB imaging and deep learning to achieve 90% accuracy in detecting *Albugo candida* infections in *Arabidopsis thaliana* before visible symptoms appear, offering a scalable solution for early plant disease management.

Crabb, G. U., Cevik, V., Chen, X. + 2 more2026-03-25📄 plant biology

Utilizing raw rapeseed press cake in foods: A case study on sensory quality and profile of selected bitter compounds in snack bars

This study demonstrates that incorporating raw rapeseed press cake into snack bars creates dose-dependent sensory attributes, particularly bitterness and astringency, which are primarily driven by specific metabolites like KSS, KSS-hexose, and goitrin rather than total protein content or dehulling treatment.

Thorsen, J. S., Bononad-Olmo, A., Toft, A. M. + 7 more2026-03-25📄 plant biology

Effector-centred proximity-dependent labelling enables the discovery of cell-surface immune receptors in plants

This study establishes effector-centred proximity-dependent labelling using TurboID as a versatile and scalable method for identifying plant cell-surface immune receptors and their guarded targets, successfully validating known interactions and discovering new ones such as SlEix1 as the functional receptor for the effector XEG1.

Cebrailoglu, N., Budak, E., Landeo Villanueva, S. + 5 more2026-03-25📄 plant biology

Characterizing key osmolytes and osmoprotectants in drought-stressed Scotch pine: a differential approach

This study introduces a differential experimental approach to distinguish between osmolytes and osmoprotectants in drought-stressed Scots pine, revealing that tryptophan, valine, and lysine act as specific osmoprotectants while organic acids like shikimic acid drive osmotic adjustment, whereas sucrose and inorganic cations do not serve as primary osmolytes.

Kartashov, A. V., Zlobin, I. E., Ivanov, Y. V. + 8 more2026-03-25📄 plant biology

Effects of agroforestry trees on microclimate and enset (Ensete ventricosum) morphophysiology in South Ethiopia

This study demonstrates that integrating scattered trees into enset homegardens in South Ethiopia significantly improves microclimate conditions and enhances most enset morphophysiological traits under canopy shade, supporting the potential of agroforestry systems to bolster climate resilience for this staple crop.

Bireda, A. Y., Garo, G., Swennen, R. + 4 more2026-03-25✓ Author reviewed 📄 plant biology

Evolutionary analysis of the exocyst in streptophytes links EXO70 diversification to dominance over SEC3 in membrane targeting

This study reveals that the diversification of the EXO70 subfamily in streptophytes, which originated during early plant terrestrialization, drove an evolutionary shift where EXO70 increasingly replaced SEC3 as the primary membrane-targeting component of the exocyst complex, thereby enabling specialized secretion pathways essential for land plant adaptation.

Haluska, S., Drdova, E. J., Drs, M. + 5 more2026-03-24📄 plant biology

Physiological responses of submerged freshwater macrophytes to multiple stressors

This meta-analysis of 124 experiments reveals that while multiple anthropogenic stressors generally cause negative physiological responses in submerged freshwater macrophytes, additive effects dominate over synergistic ones, highlighting the need for more complex, long-term studies and the use of *Stuckenia pectinata* as a model organism to improve global change predictions.

Mahdjoub, A. M., Einspanier, S., Gross, E. M. + 1 more2026-03-24📄 plant biology

Structure of the Arabidopsis receptor kinase SRF6 ectodomain determined from crystals obtained using the LRR crystallisation screen

This study reports the 1.5 Å crystal structure of the Arabidopsis SRF6 ectodomain, determined using a specialized LRR crystallization screen, and reveals that despite its structural similarity to brassinosteroid pathway receptors, it lacks canonical features and does not interact with previously proposed partners, suggesting a distinct signaling role.

Caregnato, A., Hohmann, U., Hothorn, M.2026-03-23📄 plant biology

Drought induced metabolomics of potato leaves highlight metabolic reprogramming and promising biomarkers for smart irrigation advisories

This study demonstrates that integrating metabolomics-derived biomarkers, such as proline, with satellite-based remote sensing can overcome the limitations of current irrigation advisories in heterogeneous landscapes by providing sensitive, ground-truth indicators of drought stress in potato crops for improved smart irrigation management.

Singh, P. D., Nayak, R., Dittrich, Y. + 3 more2026-03-21✓ Author reviewed 📄 plant biology